![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
MerB
Part:BBa_K4831003:Design
Designed by: Joakim Gustafsson Group: iGEM23_ABOA-Turku (2023-10-01)
MerB alkylmercury lyase from P. aeruginosa (seq. harmonized to Synechocystis sp. PCC6803)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 589
Illegal PstI site found at 143 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 589
Illegal PstI site found at 143 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 589
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 589
Illegal PstI site found at 143 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 589
Illegal PstI site found at 143
Illegal NgoMIV site found at 412 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence is modified with restriction sites for position 5 in a 7-fragment Type IIs assembly with Bbs1. The start codon ATG is also removed. The sequences codons have been harmonised for optimal translation in Synechocystis sp. PCC 6803 (changed codons: 164, GC-change 56.6 to 51.3%). The part has been checked for illegal restriction sites (Bsa1 and Sap1).
Source
Pseudomonas aeruginosa strain ARY1 organomercurial lyase (merB) gene, complete cds. https://www.ncbi.nlm.nih.gov/nuccore/FJ613641.1